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| Rodrigues, José Ariévilo Gurgel; Eloy, Ygor Raphael Gomes; Vanderlei, Edfranck de Souza Oliveira; Cavalcante, Jéssica Figueiredo; Romanos, Maria Tereza Villela; Benevides, Norma Maria Barros. |
| The antiviral potency of Caulerpa cupressoides (Chlorophyta) sulfated polysaccharidic fractions (Cc-SP1→3) associated with thrombin generation (TG) assay is unknown. This study analyzed the structure of Cc-SP1 and its effects against herpes viruses (HSV-1 and HSV-2), dengue-virus (DENV-1), human-metapneumovirus (HMPV) and adenovirus (AdV) in Vero and mosquito C6/36 cell-lines and as inhibitor of TG in 60-fold diluted human plasma using continuous system. Infrared analysis indicated ulvan containing 11% sulfate, 40.16% total sugars and 6% uronic acid. Procedures of agarose/polyacrylamide gels electrophoresis revealed two major components presenting sulfate and non SPs, with molecular dispersion ranging from 8 to > 100 kDa, respectively, as confirmed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Química de macromoléculas Bryopsidales; Marine glycans; Cytotoxicity; Viral infection; Thrombin. Bioprospecção. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28081 |
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| Rodrigues, José Ariévilo Gurgel; Barcellos, Priscila Gomes; Glauser, Bianca Fernandes; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza. |
| The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marine algae; Polyglycans; Coagulation tests; Thrombin.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463 |
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| Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza. |
| Acanthophora muscoides (Rhodophyta) has three polysulfated fractions (Am-1, Am-2 and Am-3). Am-2 displayed anti-inflammation and serpin-independent anticoagulation; however, no effect of oligomers on thrombin-generation (TG) assay has been demonstrated. This study employed mild-acid hydrolysis to obtain low-molecular-size derivatives from Am-2 and compared in vitro inhibitory effects between intact Am-2 and its hydrolysates on a TG assay. The enzymatic extract was fractionated by DEAE-cellulose that revealed Am-2 eluted with 0.75-M NaCl containing sulfate (23%), hexoses (51%) and devoid of proteins, and indicating, by one-dimension nuclear magnetic resonance, structure of galactan similar to that of extract. The depolymerization with HCl (0.02 or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências Biológicas e Ambientais Rhodophyceae; Poli-aniônicos; Depolimerização; Trombina. Química de macromoléculas Rhodophyceae; Polyanionics; Depolymerization; Thrombin. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28257 |
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| Castro, George Meredite Cunha de; Benevides, Norma Maria Barros; Cabral, Maulori Curié; Miranda, Rafael de Souza; Gomes Filho, Enéas; Rocha, Maria Valderez Ponte; Araújo, Marjory Lima Holanda. |
| The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were Y(P⁄S) = 0.48 ± 0.02 g.g−1, PP = 0.27 ±... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências da Vida Bioquímica iota-carrageenan; Cellulose; Galactose; Kinetic parameters; Fermentation; Acclimation; Sulfuric acid. Biotecnologia. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37227 |
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| Barcellos, Priscila Gomes; Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Araújo, Ianna Wivianne Fernandes de; Benevides, Norma Maria Barros; Mourão, Paulo Antônio de Souza. |
| Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ulvales; Complex glycans; Chemical analysis; Thrombosis.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916 |
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